Community Characteristics Analysis of Eukaryotic Microplankton via ITS Gene Metabarcoding Based on Environmental DNA in Lower Reaches of Qiantang River, China

Eukaryotic microplankton plays an important role in water biotic community and maintaining the stability of ecosystems. Environmental DNA metabarcoding provides opportunity to integrate traditional emerging approaches discover more new species, develop molecular indices that can be rapidly, frequently, robustly used quality assessments. In order examine assemblages eukaryotic lower reaches Qiantang River, ITS gene technology based on environmental was carried out. As a result, various species phytoplankton, fungi zooplankton were annotated on. More phylum, classes specieses phytoplankton found after compared communities taxa with obtained from morphological examination. Nevertheless, Chlorophyceae most common assemblage both identified by using these two methods, also Mesocyclops leuckarti Acanthocyclops bicuspidatus dominant Cyclopoida river. Additionally, reads proportions at three freshwater sampling sites (Tonglu, Fuyang Wenyan) decreased as temperature drop. Meanwhile, twenty on, which characteristic first researched There significant spatial differences values Chao1 index for microplankton. Cluster analysis Non-metric multidimensional scaling ordination further confirmed composition class level Jiashao-September sample had dissimilarity others.